Definiens Tissue Studio 3 provides detailed quantitative readouts on a cell-by-cell basis. Cells can be distinguished based on membrane staining or using the cell simulation feature. Length, area, width, elliptic fit, staining intensity, biomarker expression profile and dozens of additional (including custom-configured) parameters can be measured and reported per individual cell or cell compartment. This allows distinguishing different cellular populations – e.g. to address tumor heterogeneity. Compared to traditional approaches, true cell-by-cell analysis establishes a superior basis for the statistical evaluation of your results. An example of these advantages is the literal assessment of the Dako HER2/neu score including measurements of membrane staining intensity and closure per individual cell.